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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
28/04/2011 |
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Data da última atualização: |
28/04/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - -- |
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Autoria: |
LEITE, G. B.; FINARDI, N. L.; FORTES, G. R. L. |
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Afiliação: |
Epagri |
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Título: |
Use of vermiculite as a substrate and effect of light on in vitro rooting of pears, cv. Bartlett and clone OHxF 97. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, v. 26, n. 5, p. 977-982, 2002. |
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Idioma: |
Português |
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Notas: |
ISSN, 1413-7054 |
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Conteúdo: |
Agar is traditionally used to solidify medium in cultures grown on in vitro conditions.
However, it has been observed, in some cases, a poor root development, along with the non-functionality of these roots when transferred to in vivo conditions. Two trials were carried out at Embrapa Clima Temperado laboratory with pears (Pyrus sp) cv. Bartlett and OH x F97 clone), to investigate the possibility of replacing agar by vermiculite as a physical support, and to analyze the influence of light on the base of micro cuttings on
root development. Shoots were conditioned in a medium MS/3 to which was added myoinositol (100 mg.L-1; sucrose (30 g.L-1); agar (6 g.L-1) or vermiculite (8g/tube).
No growth regulator was added. The darkening treatment was accomplished by putting a black sheet on the top of the agar medium. The evaluation was performed after 30 days on the shoots being maintained on 25 °C, 2000 lux and 16 hour of photoperiod. The trial
was performed in a completely ramdomized block and the means were compared by using the Duncan test at 5% probability. Either agar or vermiculite did not affect rooting percentage. On the other hand, shoots on agar showed a higher number of roots per shoot for both ´ Bartlett` and OH x F97 clone. Root hairs were not observed on agar grown plantlets, but they were produced on vermiculite grown ones. As secondary root
is concerned, it was not observed any on agar grown shoots, whereas shoots rooted in vermiculite had around 31% and 53% of secondary root for ´Bartlett and OH x F97 clone, respectively. Darkened medium decreased the percentage of rooted plantlets from 98 to
86 and the root length from 3.5 to 2.1 cm. Menos Agar is traditionally used to solidify medium in cultures grown on in vitro conditions.
However, it has been observed, in some cases, a poor root development, along with the non-functionality of these roots when transferred to in vivo conditions. Two trials were carried out at Embrapa Clima Temperado laboratory with pears (Pyrus sp) cv. Bartlett and OH x F97 clone), to investigate the possibility of replacing agar by vermiculite as a physical support, and to analyze the influence of light on the base of micro cuttings on
root development. Shoots were conditioned in a medium MS/3 to which was added myoinositol (100 mg.L-1; sucrose (30 g.L-1); agar (6 g.L-1) or vermiculite (8g/tube).
No growth regulator was added. The darkening treatment was accomplished by putting a black sheet on the top of the agar medium. The evaluation was performed after 30 days on the shoots being maintained on 25 °C, 2000 lux and 16 hour of photoperiod. The trial
was performed in a completely ramdomized block and the means were compared by using the Duncan test at 5% probability. Either agar or vermiculite did not affect rooting percentage. On the other hand, shoots on agar showed a higher number of roots per shoot for both ´ Bartlett` and OH x F97 clone. Root hairs were not observed on agar grown plantlets, but they were produced on vermiculite grown ones. As secondary root
is concerned, it was not observed any on agar grown shoots, whereas shoots rooted in vermiculite had around 31% and 53% of secon... Mostrar Tudo |
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Palavras-Chave: |
Agar; Micropropagation; Pyrus sp; Root quality; Tissue culture. |
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Categoria do assunto: |
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Marc: |
LEADER 02241naa a2200193 a 4500
001 1075994
005 2011-04-28
008 2002 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aUse of vermiculite as a substrate and effect of light on in vitro rooting of pears, cv. Bartlett and clone OHxF 97.
260 $c2002
500 $aISSN, 1413-7054
520 $aAgar is traditionally used to solidify medium in cultures grown on in vitro conditions. However, it has been observed, in some cases, a poor root development, along with the non-functionality of these roots when transferred to in vivo conditions. Two trials were carried out at Embrapa Clima Temperado laboratory with pears (Pyrus sp) cv. Bartlett and OH x F97 clone), to investigate the possibility of replacing agar by vermiculite as a physical support, and to analyze the influence of light on the base of micro cuttings on root development. Shoots were conditioned in a medium MS/3 to which was added myoinositol (100 mg.L-1; sucrose (30 g.L-1); agar (6 g.L-1) or vermiculite (8g/tube). No growth regulator was added. The darkening treatment was accomplished by putting a black sheet on the top of the agar medium. The evaluation was performed after 30 days on the shoots being maintained on 25 °C, 2000 lux and 16 hour of photoperiod. The trial was performed in a completely ramdomized block and the means were compared by using the Duncan test at 5% probability. Either agar or vermiculite did not affect rooting percentage. On the other hand, shoots on agar showed a higher number of roots per shoot for both ´ Bartlett` and OH x F97 clone. Root hairs were not observed on agar grown plantlets, but they were produced on vermiculite grown ones. As secondary root is concerned, it was not observed any on agar grown shoots, whereas shoots rooted in vermiculite had around 31% and 53% of secondary root for ´Bartlett and OH x F97 clone, respectively. Darkened medium decreased the percentage of rooted plantlets from 98 to 86 and the root length from 3.5 to 2.1 cm.
653 $aAgar
653 $aMicropropagation
653 $aPyrus sp
653 $aRoot quality
653 $aTissue culture
773 $tCiência e Agrotecnologia, Lavras$gv. 26, n. 5, p. 977-982, 2002.
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